diff --git a/Lib/profiling/tracing/__init__.py b/Lib/profiling/tracing/__init__.py
index a6b8edf721611f..143573ff747d1b 100644
--- a/Lib/profiling/tracing/__init__.py
+++ b/Lib/profiling/tracing/__init__.py
@@ -197,7 +197,7 @@ def main():
             # in the module's namespace.
             globs = module.__dict__
             globs.update({
-                '__spec__': spec,
+                '__spec__': None,
                 '__file__': spec.origin,
                 '__name__': spec.name,
                 '__package__': None,
diff --git a/Lib/test/test_profiling/test_tracing_profiler.py b/Lib/test/test_profiling/test_tracing_profiler.py
index d09ca441d4ae46..d4bed67ffe8175 100644
--- a/Lib/test/test_profiling/test_tracing_profiler.py
+++ b/Lib/test/test_profiling/test_tracing_profiler.py
@@ -2,12 +2,14 @@
 
 import sys
 import unittest
+import subprocess
 
 # rip off all interesting stuff from test_profile
 import profiling.tracing as cProfile
 import tempfile
 import textwrap
 from test.test_profile import ProfileTest, regenerate_expected_output
+from test.support import script_helper, os_helper, SHORT_TIMEOUT
 from test.support.script_helper import assert_python_failure, assert_python_ok
 from test import support
 
@@ -170,6 +172,41 @@ class Foo:
             f.close()
             assert_python_ok('-m', "cProfile", f.name)
 
+    @unittest.skipIf(sys.platform == 'win32',
+                     'Profiler with multiprocessing can not run on win32')
+    def test_profile_multiprocessing(self):
+        test_script = '''
+import multiprocessing
+
+def worker_proc(x):
+    return x * 42
+
+def main_proc():
+    p = multiprocessing.Process(target=worker_proc, args=(10,))
+    p.start()
+    p.join()
+    print("SUCCESS")
+
+if __name__ == "__main__":
+    main_proc()
+'''
+        with os_helper.temp_dir() as temp_dir:
+            script = script_helper.make_script(
+                temp_dir, 'test_cprofile_multiprocessing', test_script
+            )
+            with script_helper.spawn_python(
+                "-m", "cProfile",
+                script,
+                stderr=subprocess.PIPE,
+                text=True
+            ) as proc:
+                proc.wait(timeout=SHORT_TIMEOUT)
+                stdout = proc.stdout.read()
+                stderr = proc.stderr.read()
+
+        self.assertIn("SUCCESS", stdout)
+        self.assertNotIn("has no attribute \'worker_proc\'", stderr)
+
 
 def main():
     if '-r' not in sys.argv:
diff --git a/Misc/NEWS.d/next/Library/2025-11-23-19-06-41.gh-issue-140729.bk_HDs.rst b/Misc/NEWS.d/next/Library/2025-11-23-19-06-41.gh-issue-140729.bk_HDs.rst
new file mode 100644
index 00000000000000..0341c43b68eab6
--- /dev/null
+++ b/Misc/NEWS.d/next/Library/2025-11-23-19-06-41.gh-issue-140729.bk_HDs.rst
@@ -0,0 +1 @@
+Set ``__main__.__spec__`` to ``None`` when running a script with :mod:`cProfile`