diff --git a/Lib/test/test_cmd_line.py b/Lib/test/test_cmd_line.py
index 3ed7a360d64e3c..6e907f8575cb83 100644
--- a/Lib/test/test_cmd_line.py
+++ b/Lib/test/test_cmd_line.py
@@ -201,6 +201,16 @@ def test_run_module_bug1764407(self):
         self.assertTrue(data.find(b'1 loop') != -1)
         self.assertTrue(data.find(b'__main__.Timer') != -1)
 
+    @support.cpython_only
+    def test_null_byte_in_interactive_mode(self):
+        # gh-140594: Fix a buffer overflow when a single NULL character is read
+        # from standard input in interactive mode. The test ensures that
+        # feeding a null byte to the interactive prompt does not crash
+        # the interpreter.
+        proc = spawn_python('-i')
+        proc.communicate(b'\x00', timeout=10)
+        self.assertEqual(proc.returncode, 0)
+
     def test_relativedir_bug46421(self):
         # Test `python -m unittest` with a relative directory beginning with ./
         # Note: We have to switch to the project's top module's directory, as per
diff --git a/Misc/NEWS.d/next/Core_and_Builtins/2025-11-02-16-23-17.gh-issue-140594.YIWUpl.rst b/Misc/NEWS.d/next/Core_and_Builtins/2025-11-02-16-23-17.gh-issue-140594.YIWUpl.rst
new file mode 100644
index 00000000000000..b263335a3d45fe
--- /dev/null
+++ b/Misc/NEWS.d/next/Core_and_Builtins/2025-11-02-16-23-17.gh-issue-140594.YIWUpl.rst
@@ -0,0 +1,2 @@
+Fix a buffer overflow when a single NULL character is read from the standard input.
+Patch by Shamil Abdulaev.
diff --git a/Parser/myreadline.c b/Parser/myreadline.c
index 64e8f5383f0602..ee77479ba7bdcc 100644
--- a/Parser/myreadline.c
+++ b/Parser/myreadline.c
@@ -344,7 +344,7 @@ PyOS_StdioReadline(FILE *sys_stdin, FILE *sys_stdout, const char *prompt)
             break;
         }
         n += strlen(p + n);
-    } while (p[n-1] != '\n');
+    } while (n == 0 || p[n-1] != '\n');
 
     pr = (char *)PyMem_RawRealloc(p, n+1);
     if (pr == NULL) {