diff --git a/src/google/adk/cli/adk_web_server.py b/src/google/adk/cli/adk_web_server.py
index 5d71591466..cf56c5f8d4 100644
--- a/src/google/adk/cli/adk_web_server.py
+++ b/src/google/adk/cli/adk_web_server.py
@@ -88,6 +88,7 @@
 from ..sessions.base_session_service import BaseSessionService
 from ..sessions.session import Session
 from ..utils.context_utils import Aclosing
+from ..utils.env_utils import is_env_enabled
 from .cli_eval import EVAL_SESSION_ID_PREFIX
 from .utils import cleanup
 from .utils import common
@@ -522,7 +523,8 @@ async def get_runner_async(self, app_name: str) -> Runner:
       return self.runner_dict[app_name]
 
     # Create new runner
-    envs.load_dotenv_for_agent(os.path.basename(app_name), self.agents_dir)
+    if not is_env_enabled("ADK_DISABLE_LOAD_DOTENV"):
+      envs.load_dotenv_for_agent(os.path.basename(app_name), self.agents_dir)
     agent_or_app = self.agent_loader.load_agent(app_name)
 
     # Instantiate extra plugins if configured
diff --git a/src/google/adk/cli/cli_tools_click.py b/src/google/adk/cli/cli_tools_click.py
index 035ddf1331..1d32c5d590 100644
--- a/src/google/adk/cli/cli_tools_click.py
+++ b/src/google/adk/cli/cli_tools_click.py
@@ -36,6 +36,7 @@
 from . import cli_deploy
 from .. import version
 from ..evaluation.constants import MISSING_EVAL_DEPENDENCIES_MESSAGE
+from ..utils.env_utils import is_env_enabled
 from .cli import run_cli
 from .fast_api import get_fast_api_app
 from .utils import envs
@@ -623,7 +624,8 @@ def cli_eval(
 
   PRINT_DETAILED_RESULTS: Prints detailed results on the console.
   """
-  envs.load_dotenv_for_agent(agent_module_file_path, ".")
+  if not is_env_enabled("ADK_DISABLE_LOAD_DOTENV"):
+    envs.load_dotenv_for_agent(agent_module_file_path, ".")
   logs.setup_adk_logger(getattr(logging, log_level.upper()))
 
   try:
diff --git a/src/google/adk/cli/fast_api.py b/src/google/adk/cli/fast_api.py
index 131213ec07..8f34db84c4 100644
--- a/src/google/adk/cli/fast_api.py
+++ b/src/google/adk/cli/fast_api.py
@@ -39,6 +39,7 @@
 from ..evaluation.local_eval_set_results_manager import LocalEvalSetResultsManager
 from ..evaluation.local_eval_sets_manager import LocalEvalSetsManager
 from ..runners import Runner
+from ..utils.env_utils import is_env_enabled
 from .adk_web_server import AdkWebServer
 from .service_registry import load_services_module
 from .utils import envs
@@ -161,7 +162,8 @@ def get_fast_api_app(
     from opentelemetry.exporter.cloud_trace import CloudTraceSpanExporter
 
     def register_processors(provider: TracerProvider) -> None:
-      envs.load_dotenv_for_agent("", agents_dir)
+      if not is_env_enabled("ADK_DISABLE_LOAD_DOTENV"):
+        envs.load_dotenv_for_agent("", agents_dir)
       if project_id := os.environ.get("GOOGLE_CLOUD_PROJECT", None):
         processor = export.BatchSpanProcessor(
             CloudTraceSpanExporter(project_id=project_id)
diff --git a/src/google/adk/cli/service_registry.py b/src/google/adk/cli/service_registry.py
index 3e7921e075..9936b16510 100644
--- a/src/google/adk/cli/service_registry.py
+++ b/src/google/adk/cli/service_registry.py
@@ -78,6 +78,7 @@ def my_session_factory(uri: str, **kwargs):
 from ..memory.base_memory_service import BaseMemoryService
 from ..sessions.base_session_service import BaseSessionService
 from ..utils import yaml_utils
+from ..utils.env_utils import is_env_enabled
 
 logger = logging.getLogger("google_adk." + __name__)
 
@@ -330,7 +331,8 @@ def _load_gcp_config(
 
   from .utils import envs
 
-  envs.load_dotenv_for_agent("", agents_dir)
+  if not is_env_enabled("ADK_DISABLE_LOAD_DOTENV"):
+    envs.load_dotenv_for_agent("", agents_dir)
 
   project = os.environ.get("GOOGLE_CLOUD_PROJECT")
   location = os.environ.get("GOOGLE_CLOUD_LOCATION")
diff --git a/src/google/adk/cli/utils/agent_loader.py b/src/google/adk/cli/utils/agent_loader.py
index d6965e5bbb..b56c7bc0bc 100644
--- a/src/google/adk/cli/utils/agent_loader.py
+++ b/src/google/adk/cli/utils/agent_loader.py
@@ -33,6 +33,7 @@
 from ...agents.base_agent import BaseAgent
 from ...apps.app import App
 from ...utils.feature_decorator import experimental
+from ...utils.env_utils import is_env_enabled
 from .base_agent_loader import BaseAgentLoader
 
 logger = logging.getLogger("google_adk." + __name__)
@@ -223,8 +224,9 @@ def _perform_load(self, agent_name: str) -> Union[BaseAgent, App]:
     if agents_dir not in sys.path:
       sys.path.insert(0, agents_dir)
 
-    logger.debug("Loading .env for agent %s from %s", agent_name, agents_dir)
-    envs.load_dotenv_for_agent(actual_agent_name, str(agents_dir))
+    if not is_env_enabled("ADK_DISABLE_LOAD_DOTENV"):
+      logger.debug("Loading .env for agent %s from %s", agent_name, agents_dir)
+      envs.load_dotenv_for_agent(actual_agent_name, str(agents_dir))
 
     if root_agent := self._load_from_module_or_package(module_base_name):
       self._record_origin_metadata(